MACS GMP Annexin V Kit
MACS GMP Annexin V Reagent recognize an antigen (Phosphatidylserine) in the plasma membrane of apoptotic as well as dead cells.
For the MACS cell depletion, cells are magnetically labeled with MACS GMP Annexin V Reagent and passed through an MS Column.
The magnetically labeled dead cells are retained in the column while the unlabeled living cells are collected in the flow-through. Using the MACS GMP Annexin V Kit, even early apoptotic cells with an intact cellular membrane are removed.
Magnetic-activated cell sorting (MACS)
- a sperm preparation technique that separates apoptotic spermatozoa from non-apoptotic spermatozoa based on the expression of phosphatidelserine (PS)
- an efficient method to select functional sperm and improve pregnancy rates
Start with 107 up to maximal 108 total spermatozoa.
Apoptosis is the programmed cell death that occurs because of the DNA fragmentation which is seen in the sperm of infertile men.
- Annexin V (AV) is a phospholipid-binding protein with a high affinity for phosphatidylserine (PS)
- Superparamagnetic microbeads (AVMB)
AV cannot pass through intact sperm membranes, and consequently, AV-conjugated superparamagnetic microbeads (AVMB) can be used to effectively separate sperm with externalized PS, a subpopulation of which may be apoptotic.
This yields two fractions:
- AV-negative (AV-, sperm presumed to be non-apoptotic with intact membranes)
- AV-positive (AV+, sperm presumed to be apoptotic with externalized PS)
The Benefit of MACS
- Avoiding apoptotic sperm during selection
- Reducing sperm DNA fragmentation levels
- Increasing fertilization rates